The Lipid Status in Patients with Ulcerative Colitis: Sphingolipids are Disease-Dependent Regulated.

ABSTRACT: The factors that contribute to the development of ulcerative colitis (UC), are still not fully identified. Disruption of the colon barrier is one of the first events leading to invasion of bacteria and activation of the immune system. The colon barrier is strongly influenced by sphingolipids. Sphingolipids impact cell-cell contacts and function as second messengers. We collected blood and colon tissue samples from UC patients and healthy controls and investigated the sphingolipids and other lipids by LC-MS/MS or LC-QTOFMS. The expression of enzymes of the sphingolipid pathway were determined by RT-PCR and immunohistochemistry. In inflamed colon tissue, the de novo-synthesis of sphingolipids is reduced, whereas lactosylceramides are increased. Reduction of dihydroceramides was due to posttr

SUBMITTER: Bazarganipour S 

PROVIDER: S-EPMC6678307 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature