Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioidesbrasiliensis and Paracoccidioides lutzii.

ABSTRACT: Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all s

SUBMITTER: Peres da Silva R 

PROVIDER: S-EPMC6678485 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature