Unique Phase Transition of Exogenous Fusion Elastin-like Polypeptides in the Solution Containing Polyethylene Glycol.

ABSTRACT: Elastin-Like polypeptides (ELPs), as well-known temperature-controlled bio-macromolecules, are widely used. However, little is known about the interactions between ELPs and macromolecules, which is an important yet neglected problem. Here, the phase transition characteristics of an ELPs-SpyCatcher fusion protein (E-C) in the presence of polyethylene glycol (PEG) in single salts (Na₂CO₃, Na₂SO₄, NaCl) solutions were investigated using a UV spectrophotometer, DLC, and fluorescence spectroscopy, and we got some interesting results. The phases transition of E-C occurred at a concentration lower than 0.5 mol/L Na₂CO₃/PEG2000, while in single Na₂CO₃ (<0.5 mol/L), the phase transition of E-C did not occur. In

SUBMITTER: Ge Z 

PROVIDER: S-EPMC6678693 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature