GroEL Gene-Based Phylogenetic Analysis of Lactobacillus Species by High-Throughput Sequencing.

ABSTRACT: Lactobacillus is a fairly diverse genus of bacteria with more than 260 species and subspecies. Many profiling methods have been developed to carry out phylogenetic analysis of this complex and diverse genus, but limitations remain since there is still a lack of comprehensive and accurate analytical method to profile this genus at species level. To overcome these limitations, a Lactobacillus-specific primer set was developed targeting a hypervariable region in the groEL gene-a single-copy gene that has undergone rapid mutation and evolution. The results showed that this methodology could accurately perform taxonomic identification of Lactobacillus down to the species level. Its detection limit was as low as 104 colony-forming units (cfu)/mL for Lactobacillus species. The assessment of detec

SUBMITTER: Xie M 

PROVIDER: S-EPMC6678851 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature