Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization.

ABSTRACT: Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S-linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We

SUBMITTER: Li J 

PROVIDER: S-EPMC6680154 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature