X-ray Micro-Computed Tomography for Nondestructive Three-Dimensional (3D) X-ray Histology.

ABSTRACT: Historically, micro-computed tomography (μCT) has been considered unsuitable for histologic analysis of unstained formalin-fixed, paraffin-embedded soft tissue biopsy specimens because of a lack of image contrast between the tissue and the paraffin. However, we recently demonstrated that μCT can successfully resolve microstructural detail in routinely prepared tissue specimens. Herein, we illustrate how μCT imaging of standard formalin-fixed, paraffin-embedded biopsy specimens can be seamlessly integrated into conventional histology workflows, enabling nondestructive three-dimensional (3D) X-ray histology, the use and benefits of which we showcase for the exemplar of human lung biopsy specimens. This technology advancement was achieved through manufacturing a first-of-kind μCT scanner for

SUBMITTER: Katsamenis OL 

PROVIDER: S-EPMC6680277 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature