Combination of Three Functionalized Temperature-Sensitive Chromatographic Materials for Serum Protein Analysis.

ABSTRACT: We have developed a methodology to capture acidic proteins, alkaline proteins, and glycoproteins separately in mouse serum using a combination of three functionalized temperature-responsive chromatographic stationary phases. The temperature-responsive polymer poly(N-isopropylacrylamide) was attached to the stationary phase, silica. The three temperature-responsive chromatographic stationary phase materials were prepared by reversible addition-fragmentation chain transfer polymerization. Alkaline, acidic, and boric acid functional groups were introduced to capture acidic proteins, alkaline proteins, and glycoproteins, respectively. The protein enrichment and release properties of the materials were examined using the acidic protein, bovine serum albumin; the alkaline protein, protami

SUBMITTER: Sun W 

PROVIDER: S-EPMC6680567 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature