Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.

ABSTRACT: Controlling and managing the degree of phenotypic diversification of microbial populations is a challenging task. This task not only requires detailed knowledge regarding diversification mechanisms but also advanced technical set-ups for the real-time analyses and control of population behaviour on single-cell level. In this work, set-up, design and operation of the so called segregostat are described which, in contrast to a traditional chemostat, allows the control of phenotypic diversification of microbial populations over time. Two exemplary case studies will be discussed, i.e. phenotypic diversification dynamics of Eschericia coli and Pseudomonas putida based on outer membrane permeabilization, emphasizing the applicability and versatility of the proposed approach. Upon nutrient limita

SUBMITTER: Sassi H 

PROVIDER: S-EPMC6680609 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature