An update of the suicide plasmid-mediated genome editing system in Corynebacterium glutamicum.

ABSTRACT: Corynebacterium glutamicum is an important industrial microorganism, but the availability of tools for its genetic modification has lagged compared to other model microorganisms such as Escherichia coli. Despite great progress in CRISPR-based technologies, the most feasible genome editing method in C. glutamicum is suicide plasmid-mediated, the editing efficiency of which is low due to high false-positive rates of sacB counter selection, and the requirement for tedious two-round selection and verification of rare double-cross-over events. In this study, an rpsL mutant conferring streptomycin resistance was harnessed for counter selection, significantly increasing the positive selection rate. More importantly, with the aid of high selection efficiencies through the use of antibiotics, namel

SUBMITTER: Wang T 

PROVIDER: S-EPMC6680612 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature