Cell density-dependent auto-inducible promoters for expression of recombinant proteins in Pseudomonas putida.

ABSTRACT: Inducible promoters such as P_lac are of limited usability for industrial protein production with Pseudomonas putida. We therefore utilized cell density-dependent auto-inducible promoters for recombinant gene expression in P. putida KT2440 based on the RoxS/RoxR Quorum Sensing (QS) system of the bacterium. To this end, genetic regions upstream of the RoxS/RoxR-regulated genes ddcA (P_{R ox132} ) and PP_3332 (P_{R ox306} ) were inserted into plasmids that mediated the expression of superfolder green fluorescent protein (sfGFP) and surface displayed mCherry, confirming their promoter functionalities. Mutation of the Pribnow box of P_{R ox306} to the σ⁷⁰ consensus sequence (P_{R ox3061} ) resulted

SUBMITTER: Meyers A 

PROVIDER: S-EPMC6680623 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature