Fluorescence-based analysis of the intracytoplasmic membranes of type I methanotrophs.

ABSTRACT: Most methanotrophic bacteria maintain intracytoplasmic membranes which house the methane-oxidizing enzyme, particulate methane monooxygenase. Previous studies have primarily used transmission electron microscopy or cryo-electron microscopy to look at the structure of these membranes or lipid extraction methods to determine the per cent of cell dry weight composed of lipids. We show an alternative approach using lipophilic membrane probes and other fluorescent dyes to assess the extent of intracytoplasmic membrane formation in living cells. This fluorescence method is sensitive enough to show not only the characteristic shift in intracytoplasmic membrane formation that is present when methanotrophs are grown with or without copper, but also differences in intracytoplasmic membrane levels at

SUBMITTER: Whiddon KT 

PROVIDER: S-EPMC6680624 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature