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Generation and Application of Fluorescent Anti-Human β2-Microglobulin VHHs via Amino Modification.

ABSTRACT: The functionalization of VHHs enables their application in almost every aspect of biomedical inquiry. Amino modification remains a common strategy for protein functionalization, though is considered to be inferior to site-specific methods and cause protein property changes. In this paper, four anti-β2M VHHs were selected and modified on the amino group by NHS-Fluo. The impacts of amino modification on these VHHs were drastically different, and among all th examples, the modified NB-1 maintained the original stability, bioactivity and homogeneity of unmodified NB-1. Specific recognition of VHHs targeting β2M detected by fluorescence imaging explored the possible applications of VHHs. Via this study, we successfully functionalized the anti-β2M VHHs through amino modification and the results are able to instruct the simple and fast functionalization of VHHs in biomedical researches.

SUBMITTER: Huang C

PROVIDER: S-EPMC6680903 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Generation and Application of Fluorescent Anti-Human β2-Microglobulin VHHs via Amino Modification.

Huang Chundong C Li Da D Ren Jun J Ji Fangling F Jia Lingyun L

Molecules (Basel, Switzerland) 20190717 14

The functionalization of VHHs enables their application in almost every aspect of biomedical inquiry. Amino modification remains a common strategy for protein functionalization, though is considered to be inferior to site-specific methods and cause protein property changes. In this paper, four anti-β2M VHHs were selected and modified on the amino group by NHS-Fluo. The impacts of amino modification on these VHHs were drastically different, and among all th examples, the modified NB-1 maintained ...[more]

PMID: 31319525

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