Regional Analysis of the Brain Transcriptome in Mice Bred for High and Low Methamphetamine Consumption.

ABSTRACT: Transcriptome profiling can broadly characterize drug effects and risk for addiction in the absence of drug exposure. Modern large-scale molecular methods, including RNA-sequencing (RNA-Seq), have been extensively applied to alcohol-related disease traits, but rarely to risk for methamphetamine (MA) addiction. We used RNA-Seq data from selectively bred mice with high or low risk for voluntary MA intake to construct coexpression and cosplicing networks for differential risk. Three brain reward circuitry regions were explored, the nucleus accumbens (NAc), prefrontal cortex (PFC), and ventral midbrain (VMB). With respect to differential gene expression and wiring, the VMB was more strongly affected than either the PFC or NAc. Coexpression network connectivity was higher in the low MA drinking

SUBMITTER: Hitzemann R 

PROVIDER: S-EPMC6681006 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature