Clinical validation of a gene expression signature that differentiates benign nevi from malignant melanoma.

ABSTRACT:

Background

Histopathologic examination is sometimes inadequate for accurate and reproducible diagnosis of certain melanocytic neoplasms. As a result, more sophisticated and objective methods have been sought. The goal of this study was to identify a gene expression signature that reliably differentiated benign and malignant melanocytic lesions and evaluate its potential clinical applicability. Herein, we describe the development of a gene expression signature and its clinical validation using multiple independent cohorts of melanocytic lesions representing a broad spectrum of histopathologic subtypes.

Methods

Using quantitative reverse-transcription polymerase chain reaction (PCR) on a selected set of 23 differentially expressed genes, and by applying a threshold value and w

SUBMITTER: Clarke LE 

PROVIDER: S-EPMC6681167 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature