NMR Based Metabolomics Comparison of Different Blood Sampling Techniques in Awake and Anesthetized Rats.

ABSTRACT: The composition of body fluids has become one of the most commonly used methods for diagnosing various diseases or monitoring the drug responses, especially in serum/plasma. It is therefore vital for investigators to find an appropriate way to collect blood samples from laboratory animals. This study compared blood samples collected from different sites using the NMR based metabolomics approach. Blood samples were collected from the saphenous vein (awake state), tail vein (awake and anesthetized states after administration of sevoflurane or pentobarbital) and the inferior thoracic vena cava (ITVC, anesthetized state). These approaches from the saphenous and tail veins have the potential to enable the collection of multiple samples, and the approach from ITVC is the best method for the coll

SUBMITTER: Du H 

PROVIDER: S-EPMC6681412 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature