Bone Marrow-Derived CD44⁺ Cells Migrate to Tissue-Engineered Constructs via SDF-1/CXCR4-JNK Pathway and Aid Bone Repair.

ABSTRACT:

Background and aims

Host-derived cells play crucial roles in the regeneration process of tissue-engineered constructs (TECs) during the treatment of large segmental bone defects (LSBDs). However, their identity, source, and cell recruitment mechanisms remain elusive.

Methods

A complex model was created using mice by combining methods of GFP⁺ bone marrow transplantation (GFP-BMT), parabiosis (GFP⁺-BMT and wild-type mice), and femoral LSBD, followed by implantation of TECs or DBM scaffolds. Postoperatively, the migration of host BM cells was detected by animal imaging and immunofluorescent staining. Bone repair was evaluated by micro-CT. Signaling pathway repressors including AMD3100 and SP600125 associated with the migration of BM CD44⁺ cells

SUBMITTER: Lu Y 

PROVIDER: S-EPMC6681616 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature