⁶⁴Cu-Labeled Ubiquitin for PET Imaging of CXCR4 Expression in Mouse Breast Tumor.

ABSTRACT: Ubiquitin has been recently identified as a chemokine receptor 4 (CXCR4) natural ligand, offering great potential for positron emission computed tomography (PET) imaging of CXCR4 expression. This study reports the preparation and evaluation of (⁶⁴Cu)-radiolabeled ubiquitin for CXCR4 imaging. The ubiquitin was first fused with a C-terminal GGCGG sequence, and the resulting recombinant ubiquitin derivative UbCG4 was then functionalized with the trans-cyclooctene (TCO) moiety via thiol-maleimide click reaction, followed by ⁶⁴Cu-radiolabeling through the TCO/Tz (tetrazine)-based Diels-Alder click reaction. In the prepared in vitro studies, the prepared (⁶⁴Cu)-UbCG4 showed significantly higher specific uptakes in the 4T1 breast cancer cells compared with

SUBMITTER: Li H 

PROVIDER: S-EPMC6682141 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature