Association between cell growth and vancomycin resistance in clinical community-associated methicillin-resistant Staphylococcus aureus.

ABSTRACT:

Objectives

We investigated the association between the rate of cell growth and vancomycin (VAN) resistance by comparing the genomic and phenotypic characteristics of different sized colonies isolated from a single origin.

Methods

Vancomycin-intermediate Staphylococcus aureus (VISA) strain TMUS2136 was isolated from the pus of a patient with a brain abscess and a chronic subdural abscess. This strain grew slowly and formed colonies poorly on agar plates within 24 h of incubation. However, variously sized colonies were observed after 48 h of incubation. We isolated five strains from different sized colonies and compared their VAN susceptibility and genomic sequences using next-generation sequencing.

Results

The five strains showed different rates of growth and su

SUBMITTER: Yamaguchi T 

PROVIDER: S-EPMC6682180 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature