A cell-engineered system to assess tumor cell sensitivity to CD8⁺ T cell-mediated cytotoxicity.

ABSTRACT: In vitro assays that evaluate CD8⁺ T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.

SUBMITTER: Nelson N 

PROVIDER: S-EPMC6682348 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature