Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.

ABSTRACT: The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-specific, sub-nuclear and dynamic processes. We provide a pointer for utilising high-to-super-resolution microscopy and image processing to probe plant nuclear architecture in 3D at the best possible spatial and temporal resolution and at quantitative and cell-specific levels. High-end im

SUBMITTER: Dumur T 

PROVIDER: S-EPMC6682351 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature