Single-neuron perturbations reveal feature-specific competition in V1.

ABSTRACT: The computations performed by local neural populations, such as a cortical layer, are typically inferred from anatomical connectivity and observations of neural activity. Here we describe a method-influence mapping-that uses single-neuron perturbations to directly measure how cortical neurons reshape sensory representations. In layer 2/3 of the primary visual cortex (V1), we use two-photon optogenetics to trigger action potentials in a targeted neuron and calcium imaging to measure the effect on spiking in neighbouring neurons in awake mice viewing visual stimuli. Excitatory neurons on average suppressed other neurons and had a centre-surround influence profile over anatomical space. A neuron's influence on its neighbour depended on their similarity in activity. Notably, neurons suppressed

SUBMITTER: Chettih SN 

PROVIDER: S-EPMC6682407 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature