The Systemin Signaling Cascade As Derived from Time Course Analyses of the Systemin-responsive Phosphoproteome.

ABSTRACT: Systemin is a small peptide with important functions in plant wound response signaling. Although the transcriptional responses of systemin action are well described, the signaling cascades involved in systemin perception and signal transduction at the protein level are poorly understood. Here we used a tomato cell suspension culture system to profile phosphoproteomic responses induced by systemin and its inactive Thr17Ala analog, allowing us to reconstruct a systemin-specific kinase/phosphatase signaling network. Our time-course analysis revealed early phosphorylation events at the plasma membrane, such as dephosphorylation of H⁺-ATPase, rapid phosphorylation of NADPH-oxidase and Ca²⁺-ATPase. Later responses involved transient phosphorylation of small GTPases, vesicle

SUBMITTER: Haj Ahmad F 

PROVIDER: S-EPMC6683004 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature