A Label-free Mass Spectrometry Method to Predict Endogenous Protein Complex Composition.

ABSTRACT: Information on the composition of protein complexes can accelerate mechanistic analyses of cellular systems. Protein complex composition identifies genes that function together and provides clues about regulation within and between cellular pathways. Cytosolic protein complexes control metabolic flux, signal transduction, protein abundance, and the activities of cytoskeletal and endomembrane systems. It has been estimated that one third of all cytosolic proteins in leaves exist in an oligomeric state, yet the composition of nearly all remain unknown. Subunits of stable protein complexes copurify, and combinations of mass-spectrometry-based protein correlation profiling and bioinformatic analyses have been used to predict protein complex subunits. Because of uncertainty regarding the power

SUBMITTER: McBride Z 

PROVIDER: S-EPMC6683005 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature