Urinary DNA methylation biomarkers for prediction of prostate cancer upgrading and upstaging.

ABSTRACT:

Background

Significant numbers of prostate cancer (PCa) patients experience tumour upstaging and upgrading in surgical specimens that cause serious problems in timely and proper selection of the treatment strategy. This study was aimed at the evaluation of a set of established epigenetic biomarkers as a noninvasive tool for more accurate PCa categorization before radical prostatectomy (RP).

Methods

Quantitative methylation-specific PCR was applied for the methylation analysis of RARB, RASSF1, and GSTP1 in 514 preoperatively collected voided or catheterized urine samples from the single-centre cohort of 1056 treatment-naïve PCa patients who underwent RP. The rates of biopsy upgrading and upstaging were analysed in the whole cohort.

Results

Pathological examination of R

SUBMITTER: Bakavicius A 

PROVIDER: S-EPMC6683454 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature