RNA methylomes reveal the m⁶A-mediated regulation of DNA demethylase gene SlDML2 in tomato fruit ripening.

ABSTRACT:

Background

Methylation of nucleotides, notably in the forms of 5-methylcytosine (5mC) in DNA and N⁶-methyladenosine (m⁶A) in mRNA, carries important information for gene regulation. 5mC has been elucidated to participate in the regulation of fruit ripening, whereas the function of m⁶A in this process and the interplay between 5mC and m⁶A remain uncharacterized.

Results

Here, we show that mRNA m⁶A methylation exhibits dynamic changes similar to DNA methylation during tomato fruit ripening. RNA methylome analysis reveals that m⁶A methylation is a prevalent modification in the mRNA of tomato fruit, and the m⁶A sites are enriched around the stop codons and within the 3' untranslated regions. In the frui

SUBMITTER: Zhou L 

PROVIDER: S-EPMC6683476 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature