Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants.

ABSTRACT:

Background

The effective use of mutant populations for reverse genetic screens relies on the population-wide characterization of the induced mutations. Genome- and population-wide characterization of the mutations found in fast neutron populations has been hindered, however, by the wide range of mutations generated and the lack of affordable technologies to detect DNA sequence changes. In this study, we therefore aimed to test whether genotyping-by-sequencing (GBS) technology could be used to characterize copy number variation (CNV) induced by fast neutrons in a soybean mutant population.

Results

We called CNVs from GBS data in 79 soybean mutants and assessed the sensitivity and precision of this approach by validating our results against array comparative genomic hybridizat

SUBMITTER: Lemay MA 

PROVIDER: S-EPMC6683502 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature