Rapid Aldosterone-Mediated Signaling in the DCT Increases Activity of the Thiazide-Sensitive NaCl Cotransporter.

ABSTRACT:

Background

The NaCl cotransporter NCC in the kidney distal convoluted tubule (DCT) regulates urinary NaCl excretion and BP. Aldosterone increases NaCl reabsorption via NCC over the long-term by altering gene expression. But the acute effects of aldosterone in the DCT are less well understood.

Methods

Proteomics, bioinformatics, and cell biology approaches were combined with animal models and gene-targeted mice.

Results

Aldosterone significantly increases NCC activity within minutes in vivo or ex vivo. These effects were independent of transcription and translation, but were absent in the presence of high potassium. In vitro, aldosterone rapidly increased intracellular cAMP and inositol phosphate accumulation, and altered phosphorylation of

SUBMITTER: Cheng L 

PROVIDER: S-EPMC6683706 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature