Erythrocyte Adenosine A2B Receptor-Mediated AMPK Activation: A Missing Component Counteracting CKD by Promoting Oxygen Delivery.

ABSTRACT:

Background

Oxygen deprivation or hypoxia in the kidney drives CKD and contributes to end organ damage. The erythrocyte's role in delivery of oxygen (O₂) is regulated by hypoxia, but the effects of CKD are unknown.

Methods

We screened all of the metabolites in the whole blood of mice infused with angiotensin II (Ang II) at 140 ng/kg per minute up to 14 days to simulate CKD and compared their metabolites with those from untreated mice. Mice lacking a receptor on their erythrocytes called ADORA2B, which increases O₂ delivery, and patients with CKD were studied to assess the role of ADORA2B-mediated O₂ delivery in CKD.

Results

Untargeted metabolomics showed increased production of 2,3-biphosphoglycerate (2,3-BPG), an erythrocyte-specific m

SUBMITTER: Peng Z 

PROVIDER: S-EPMC6683722 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature