Deletion of Kir5.1 Impairs Renal Ability to Excrete Potassium during Increased Dietary Potassium Intake.

ABSTRACT:

Background

The basolateral potassium channel in the distal convoluted tubule (DCT), comprising the inwardly rectifying potassium channel Kir4.1/Kir5.1 heterotetramer, plays a key role in mediating the effect of dietary potassium intake on the thiazide-sensitive NaCl cotransporter (NCC). The role of Kir5.1 (encoded by Kcnj16) in mediating effects of dietary potassium intake on the NCC and renal potassium excretion is unknown.

Methods

We used electrophysiology, renal clearance, and immunoblotting to study Kir4.1 in the DCT and NCC in Kir5.1 knockout (Kcnj16^-/- ) and wild-type (Kcnj16^+/+ ) mice fed with normal, high, or low potassium diets.

Results

We detected a 40-pS and 20-pS potassium channel in the basolateral membrane of th

SUBMITTER: Wu P 

PROVIDER: S-EPMC6683724 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature