Incomplete influenza A virus genomes occur frequently but are readily complemented during localized viral spread.

ABSTRACT: Segmentation of viral genomes into multiple RNAs creates the potential for replication of incomplete viral genomes (IVGs). Here we use a single-cell approach to quantify influenza A virus IVGs and examine their fitness implications. We find that each segment of influenza A/Panama/2007/99 (H3N2) virus has a 58% probability of being replicated in a cell infected with a single virion. Theoretical methods predict that IVGs carry high costs in a well-mixed system, as 3.6 virions are required for replication of a full genome. Spatial structure is predicted to mitigate these costs, however, and experimental manipulations of spatial structure indicate that local spread facilitates complementation. A virus entirely dependent on co-infection was used to assess relevance of IVGs in vivo. This virus g

SUBMITTER: Jacobs NT 

PROVIDER: S-EPMC6684657 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature