TERT expression is susceptible to BRAF and ETS-factor inhibition in BRAF^V600E/TERT promoter double-mutated glioma.

ABSTRACT: The BRAF gene and the TERT promoter are among the most frequently altered genomic loci in low-grade (LGG) and high-grade-glioma (HGG), respectively. The coexistence of BRAF and TERT promoter aberrations characterizes a subset of aggressive glioma. Therefore, we investigated interactions between those alterations in malignant glioma. We analyzed co-occurrence of BRAF^V600E and TERT promoter mutations in our clinical data (n = 8) in addition to published datasets (n = 103) and established a BRAF^V600E-positive glioma cell panel (n = 9) for in vitro analyses. We investigated altered gene expression, signaling events and TERT promoter activity upon BRAF- and E-twenty-six (ETS)-factor inhibition by qRT-PCR, chromatin immunoprecipitation (ChIP), Western blots and luciferase r

SUBMITTER: Gabler L 

PROVIDER: S-EPMC6685154 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature