Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli.

ABSTRACT:

Background

Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. Acetyl-CoA can be produced from glucose, acetate, or fatty acids via metabolic pathways in Escherichia coli. Although glucose is an efficient carbon source for acetyl-CoA production, the pathway from acetate to acetyl-CoA is the shortest and fatty acids can produce acetyl-CoA through fatty acid oxidation along with abundant NADH and FADH₂. In this study, metabolically engineered E. coli strains for efficiently supplying acetyl-CoA from glucose, acetate, and fatty acid were constructed and applied in one-step biosynthesis of N-acetylglutamate (NAG) from glutamate and acetyl-CoA.

Results

A metabolically eng

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6685171 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature