Assessment of NAD⁺metabolism in human cell cultures, erythrocytes, cerebrospinal fluid and primate skeletal muscle.

ABSTRACT: The reduction-oxidation state of NAD⁺/NADH is critical for cellular health with NAD⁺ and its metabolites playing critical roles in aging and pathologies. Given the inherent autooxidation of reduced dinucleotides (i.e. NADH/NADPH), and the well-established differential stability, the accurate measurement of NAD⁺ and its metabolites is technically challenging. Moreover, sample processing, normalization and measurement strategies can profoundly alter results. Here we developed a rapid and sensitive liquid chromatography mass spectrometry-based method to quantify the NAD⁺ metabolome with careful consideration of these intrinsic chemical instabilities. Utilizing this method we assess NAD⁺ metabolite stabilities and determine the presence an

SUBMITTER: Demarest TG 

PROVIDER: S-EPMC6685425 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature