Phenotypic and functional analysis of malignant mesothelioma tumor-infiltrating lymphocytes.

ABSTRACT: Given the growing interest and promising preliminary results of immunotherapy in malignant pleural mesothelioma (MPM), it has become important to more fully understand the immune landscape in this tumor. This may be especially relevant in deciding who might benefit most from checkpoint blockade or agonist antibody therapy. Since the phenotype of tumor infiltrating lymphocytes (TILs) in MPM has not been fully described and their function has not been carefully assessed, we collected fresh tumor and blood from 22 patients undergoing surgical resection and analysed single cell suspensions by flow cytometry. The functionality of TILs was assessed by measurement of cytokine expression (IFN-γ) following overnight stimulation ex vivo. Results showed low numbers of CD8+ TILs whose function

SUBMITTER: Klampatsa A 

PROVIDER: S-EPMC6685523 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature