Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation.

ABSTRACT:

Background

Proteins in human milk are essential and known to support the growth, development, protection, and health of the newborn. These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa. Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.

Objective

This study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.

Design

A tandem mass spectrometry method was applied to 231 bre

SUBMITTER: Goonatilleke E 

PROVIDER: S-EPMC6686052 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature