Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants.

ABSTRACT: Plant expression systems have proven to be exceptional in producing high-value complex polymeric proteins such as secretory IgAs (SIgAs). However, polymeric protein production requires the expression of multiple genes, which can be transformed as single or multiple T-DNA units to generate stable transgenic plant lines. Here, we evaluated four strategies to stably transform multiple genes and to obtain high expression of all components. Using the in-seed expression of a simplified secretory IgA (sSIgA) as a reference molecule, we conclude that it is better to spread the genes over two T-DNAs than to contain them in a single T-DNA, because of the presence of homologous recombination events and gene silencing. These T-DNAs can be cotransformed to obtain transgenic plants in one transformation

SUBMITTER: Palaci J 

PROVIDER: S-EPMC6686127 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature