Development of an efficient root transgenic system for pigeon pea and its application to other important economically plants.

ABSTRACT: For non-model plants, functional characterization of genes is still hampered by lack of efficient stable transformation procedures. Here, we report a simple, fast and efficient transformation technique with Agrobacterium rhizogenes for generating stable transgenic roots in living plants to facilitate functional studies in vivo. We showed that injection of A. rhizogenes into stems of various plant species lead to stable transgenic root generation, which can sustain plant growth after the original, non-transgenic roots were cut off. A transformation system was established for pigeon pea, a major woody food crop, after optimizing the selection of A. rhizogenes strains, bacterium concentration, injection position and seedling age. RT-PCR and fluorescence observation indicated a transgenic root

SUBMITTER: Meng D 

PROVIDER: S-EPMC6686128 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature