Genome-wide profiling reveals alternative polyadenylation of mRNA in human non-small cell lung cancer.

ABSTRACT:

Background

Lung cancer is the second most common cancer with an extremely poor overall survival rate. Post-transcriptional regulation of gene expression play many important roles in human cancer, and one of the potential mechanisms underlying this is alternative mRNA maturation at its 3' untranslated regions (3'-UTRs).

Methods

Cancer tissues and paired adjacent normal lung tissues from 26 patients diagnosed with non-small cell lung cancer (NSCLC) were analyzed by in vitro transcription-sequencing alternative polyadenylation sites (IVT-SAPAS). 41,773,101 reads in average were obtained from each paired sample. A potential regulation of Cleavage Stimulation Factor Subunit 2 (CSTF2) on 3'UTR length of genes was tested in H460 cells.

Results

1439 (10.26%) genes showed up-r

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6686416 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature