High-resolution melting of multiple barcode amplicons for plant species authentication.

ABSTRACT: In recent years, species identification in herbs has attracted considerable attention due to several cases of fraud; hence inexpensive high-throughput authentication methods are highly welcomed. Species authentication is often performed through DNA analysis and several specific regions (barcodes) are considered suitable. Each barcode (Bar) possesses different qualities in terms of universality and discrimination power. A multiplexed format where information can be extracted simultaneously from several barcode regions is seemingly appropriate to ensure the power of both universality and discrimination. In this approach, we amplified DNA from five different barcode regions in a multiplexed PCR format followed by high-resolution melting (HRM). This multiplexed Bar-HRM approach was first appli

SUBMITTER: Ballin NZ 

PROVIDER: S-EPMC6686639 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature