Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species.

ABSTRACT:

Background

Recently, Tie2/TEK receptor tyrosine kinase (Tie2 or syn. angiopoietin-1 receptor) positive nucleus pulposus progenitor cells were detected in human, cattle, and mouse. These cells show remarkable multilineage differentiation capacity and direct correlation with intervertebral disc (IVD) degeneration and are therefore an interesting target for regenerative strategies. Nevertheless, there remains controversy over the presence and function of these Tie2⁺ nucleus pulposus cells (NPCs), in part due to the difficulty of identification and isolation.

Purpose

Here, we present a comprehensive protocol for sorting of Tie2⁺ NPCs from human, canine, bovine, and murine IVD tissue. We describe enhanced conditions for expansion and an optimized fluorescenc

SUBMITTER: Sakai D 

PROVIDER: S-EPMC6686801 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature