Discogenic cell transplantation directly from a cryopreserved state in an induced intervertebral disc degeneration canine model.

ABSTRACT: A multitude of studies has indicated the potential of cell therapy as a method for intervertebral disc (IVD) regeneration. Transplantation of a variety of cells has been assessed and shown capable of deterring the rate of degeneration in animal models and in human clinical trials. In this study, a novel approach using human discogenic nucleus pulposus cells directly from their cryopreserved state was assessed. In an established canine disc degeneration model, the degeneration process was evaluated in IVDs receiving precultured discogenic cells, thawed-only discogenic cells, and a saline sham injection after induction of degeneration. Degeneration progression was followed over time by the evaluation of the disc height index (DHI). Finally, after 12 weeks, the manipulated and control discs w

SUBMITTER: Hiraishi S 

PROVIDER: S-EPMC6686803 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature