UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae.

ABSTRACT: We determined the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 min after recovery in growth medium. Laser-irradiated cells were viable, and the transcriptional response was transient, with over 400 genes differentially expressed at T15 or T30, returning to basal level transcription by T60. Identification of transcripts exhibiting enhanced differential expression that were unique to UV laser-irradiation were identified by imposing a stringent significance cut-off (P < 0.05, log₂ difference >2) then filtering out genes known as environmental stress response (ESR) genes. Using these rigorous criteria, 56 genes were differentially expressed at T15; at T30 differe

SUBMITTER: Hauser M 

PROVIDER: S-EPMC6686910 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature