Fragmentation of Pooled PCR Products for Highly Multiplexed TILLING.

ABSTRACT: Improvements to massively parallel sequencing have allowed the routine recovery of natural and induced sequence variants. A broad range of biological disciplines have benefited from this, ranging from plant breeding to cancer research. The need for high sequence coverage to accurately recover single nucleotide variants and small insertions and deletions limits the applicability of whole genome approaches. This is especially true in organisms with a large genome size or for applications requiring the screening of thousands of individuals, such as the reverse-genetic technique known as TILLING. Using PCR to target and sequence chosen genomic regions provides an attractive alternative as the vast reduction in interrogated bases means that sample size can be dramatically increased through ampl

SUBMITTER: Tramontano A 

PROVIDER: S-EPMC6686939 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature