Simultaneous quantification of lidocaine and prilocaine in human plasma by LC-MS/MS and its application in a human pharmacokinetic study.

ABSTRACT:

Objective

The aim of the work was to develop and validate a simple, sensitive and selective Liquid chromatography with Mass spectroscopic method for simultaneous quantification of lidocaine and prilocaine in human plasma.

Design and methods

Analytes and the internal standards from human plasma were extracted by using solid- phase extraction technique using Waters Oasis® HLB 1 ​cc (30 ​mg) cartridges. The reconstituted samples were chromatographed on Phenomenex Kinetex EVO 4.6*100 ​mm 2.6 μ 100A column by using a mixture of acetonitrile and 5 ​mM ammonium acetate buffer (80:20, v/v) as the mobile phase at a flow rate of 0.6 ​mL/min.

Results

The method was validated over the concentration range of 0.10-201.80 ng/mL for lidocaine and 0.10-201.66 ng/mL for prilocaine. The

SUBMITTER: Rao Yadlapalli SS 

PROVIDER: S-EPMC6687230 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature