Real-Time Imaging of Vaccine Biodistribution Using Zwitterionic NIR Nanoparticles.

ABSTRACT: Efficient and timely delivery of vaccine antigens to the secondary lymphoid tissue is crucial to induce protective immune responses by vaccination. However, determining the longitudinal biodistribution of injected vaccines in the body has been a challenge. Here, the near-infrared (NIR) fluorescence imaging is reported that can efficiently enable the trafficking and biodistribution of vaccines in real time. Zwitterionic NIR fluorophores are conjugated on the surface of model vaccines and tracked the fate of bioconjugated vaccines after intradermal administration. Using an NIR fluorescence imaging system, it is possible to obtain time-course imaging of vaccine trafficking through the lymphatics, observing notable uptake in lymph nodes with minimal nonspecific tissue interactions. Flow cytome

SUBMITTER: Katagiri W 

PROVIDER: S-EPMC6687515 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature