Removing melatonin receptor type 1 signaling leads to selective leptin resistance in the arcuate nucleus.

ABSTRACT: Recent studies have highlighted the involvement of melatonin in the regulation of energy homeostasis. In this study, we report that mice lacking melatonin receptor 1 (MT₁ KO) gained more weight, had a higher cumulative food intake, and were more hyperphagic after fasting compared to controls (WT). In response to a leptin injection, MT₁ KO mice showed a diminished reduction in body weight and food intake. To evaluate hypothalamic leptin signaling, we tested leptin-induced phosphorylation of the signal transducer and activator of transcription 3 (STAT3). Leptin failed to induce STAT3 phosphorylation in MT₁ KO mice beyond levels observed in mice injected with phosphate-buffered saline (PBS). Furthermore, STAT3 phosphorylation within the arcuate nucleus (ARH) w

SUBMITTER: Buonfiglio D 

PROVIDER: S-EPMC6687516 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature