A Conserved Allosteric Pathway in Tyrosine Kinase Regulation.

ABSTRACT: An autoinhibitory network of hydrogen bonds located at the kinase hinge (referred to as the "molecular brake") regulates the activity of several receptor tyrosine kinases. The mechanism whereby mutational disengagement of the brake allosterically activates the kinase in human disease is incompletely understood. We used a combination of NMR, bioinformatics, and molecular dynamics simulation to show that mutational disruption of the molecular brake triggers localized conformational perturbations that propagate to the active site. This entails changes in interactions of an isoleucine, one of three hydrophobic residues that lock the phenylalanine of the DFG motif in an inactive conformation. Structural analysis of tyrosine kinases provides evidence that this allosteric control mechanism is sha

SUBMITTER: Marsiglia WM 

PROVIDER: S-EPMC6687525 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature