Transcriptome analysis after PPARγ activation in human meibomian gland epithelial cells (hMGEC).

ABSTRACT:

Purpose

PPARγ plays a critical role in the maturation of immortalized human meibomian gland epithelial cells (hMGEC). To further understand the molecular changes associated with meibocyte differentiation, we analyzed transcriptome profiles from hMGEC after PPARγ activation.

Methods

Three sets of cultivated hMGEC with or without exposure to PPARγ agonist, rosiglitazone were used for RNA-seq analysis. RNA was isolated and processed to generate 6 libraries. The libraries were then sequenced and mapped to the human reference genome, and the expression results were gathered as reads per length of transcript in kilobases per million mapped reads (RPKM) values. Differential gene expression analyses were performed using DESeq2 and NOISeq. Gene ontology enrichment analysis (GOEA) was

SUBMITTER: Kim SW 

PROVIDER: S-EPMC6687577 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature