Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency.

ABSTRACT: Methionine dependency of tumor growth, although not well-understood, is detectable by ¹¹C-methionine positron emission tomography and may contribute to the aggressivity of glioblastomas (GBM) and meningiomas. Cytosolic folate cycle is required for methionine synthesis. Its dysregulation may influence cell reprogramming towards pluripotency. We evaluated methionine-dependent growth of monolayer (ML) cells and stem cell-like tumor spheres (TS) derived from 4 GBM (U251, U87, LN299, T98G) and 1 meningioma (IOMM-LEE) cell lines. Our data showed that for all cell lines studied, exogenous methionine is required for TS formation but not for ML cells proliferation. Furthermore, for GBM cell lines, regardless of the addition of folate cycle substrates (folic acid and formate), the level o

SUBMITTER: Zgheib R 

PROVIDER: S-EPMC6687714 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature