Flow Plex-A tool for unbiased comprehensive flow cytometry data analysis.

ABSTRACT:

Introduction

The information content of multiparametric flow cytometry experiments is routinely underexploited given the paucity of adequate tools for unbiased comprehensive data analysis that can be applied successfully and independently by immunologists without computational training.

Methods

We aimed to develop a tool that allows straightforward access to the entire information content of any given flow cytometry panel for immunologists without special computational expertise. We used a data analysis approach which accounts for all mathematically possible combinations of markers in a given panel, coded the algorithm and applied the method to mined and self-generated data sets.

Results

We developed Flow Plex, a straightforward computational tool that allows unrestri

SUBMITTER: Nowatzky J 

PROVIDER: S-EPMC6688088 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature